pixIO™ iPSC-derived Human Intestinal Organoids

Physiologically relevant, multi-lineage intestinal organoids for absorption, metabolism, and toxicity studies.

Cryopreserved vials containing 250 organoids each — ready-to-culture 3D format
Contain functional enterocytes, goblet, Paneth, enteroendocrine, and stem cells expressing LGR5, VILLIN, MUC2, CHGA, and CYP3A4
Available in 3D and monolayer formats for barrier integrity, transporter (ABCB1/MDR1), and drug permeability assays

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pixl.bio intestinal organoids (pixIO) are iPSC-derived 2D or 3D cultures that replicate the cellular complexity and physiology of the human gut epithelium.

They contain the full repertoire of intestinal cell types: enterocytes, goblet cells, Paneth cells, enteroendocrine cells, and stem cells, arranged in a crypt–villus architecture.These models recapitulate nutrient transport, barrier integrity, and drug metabolism pathways, providing a reproducible and scalable alternative to primary intestinal tissue. With robust expression of ADME transporters, CYP450 enzymes, and detoxification pathways, pixIO are ideally suited for studies in drug absorption, efflux transport, safety assessment, and infectious disease modeling.

Cellular fidelity

include enterocytes, goblet, Paneth, enteroendocrine, and stem cells.

Crypt-villus morphology

with barrier integrity for permeability and transport assays

Comparable transcriptional profile to primary intestine (RNA-seq data), distinct from carcinoma lines.

Expression of key gut markers (LGR5, CHGA, VILLIN, MUC2, LYZ, KRT19) at mRNA and protein levels.

Functional metabolism and transport

CYP3A4 induction by rifampicin/vitamin D3; MDR1 (ABCB1) efflux activity with verapamil sensitivity.

Detoxification capacity

GST activity comparable to human reference models.

Reproducibility

standardized organoid product from wild-type iPSC donors with normal karyotype.

Flexible formats

wild-type, CRISPR-engineered, or disease-specific donors

Technical Data & Functional Validation

pixIO Intestinal Organoid Morphology

pixIO morphology after two (left) and eight (right) passages encapsulated in Matrigel

Key Intestinal Cell Markers

pixlbio intestinal organoids (pixIO) demonstrate comparable gene expression levels of key intestinal markers (LGR5, CHGA, VILLIN, MUC2, LYZ, KRT19) to human small intestine tissue.

Key Drug Metabolism and Transporter Markers

Heat map following bulk RNA-sequencing depicting the expression levels of key intestinal markers expressed in different cell populations (enterocytes, Paneth cells, Goblet cells, stem cells, enteroendocrine cells), revealing a similar transcriptional profile between pixlbio pixIO intestinal organoids, primary human small intestine, and primary human colon tissues. Distinct transcriptional differences are observed between the first three intestinal models and Caco2 carcinoma cells.

Functional CYP450 Induction and Detoxification

CYP3A4 mRNA induction in pixIO following 72 hours of treatment with vehicle, 50μM Rifampicin, or 100nM vitamin D3 (CYP3A4 inducers) (upper). pixIO intestinal organoids demonstrate functional detoxifcation pathways, as shown by comparable Glutathione-S-Transferase (GST) activity to that observed in colorectal adenocarcinoma Caco2 cells (lower). Data are presented as mean±SEM of n=2-3 independent experiments.

pixlbio iPSC-derived intestinal organoids (pixIO) for drug metabolsim, absorption and safety assessment

Case Study

October 15, 2025

pixlbio iPSC-derived intestinal organoids (pixIO) for drug metabolsim, absorption and safety assessment

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