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Catalog Numbers: HEP-003, HEP-059, HEP-004

pixHep iPSC-derived Human Hepatocytes

Human iPSC-derived hepatocytes for predictive disease modeling and toxicity studies.

  • Cryopreserved, functionally mature hepatocytes comparable to primary cells
  • Proven performance in DILI, MASLD, and metabolism assays
  • Consistent, scalable production with 3 available donors off-the-shelf (5 x 10^6 viable cells guaranteed per vial)
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Item
pixHep-003 iPSC-derived Hepatocyte Donor 1, Male (Wild Type)
Price
£ 1,700 
0

pixHep™ are iPSC-derived hepatocytes with mature morphology, functional CYP activity, and stable performance for 30+ days in culture.

They are available in wild-type, disease-specific, or CRISPR-edited formats, enabling precise modelling of metabolism, toxicity, and disease pathways.

Stable Phase I/II enzyme activity for over a month.

Maintains metabolic function for long-term studies, enabling chronic toxicity and metabolite profiling.

ASGR1 is capitalised

Displays native transporter activity for realistic drug absorption and liver-specific distribution studies.

Predictive DILI response matchint clinical outcomes.

Accurately identifies human-relevant drug-induced liver injury during preclinical screening.

Custom genetic variants for mechanistic studies.

CRISPR-engineered pixHeps model disease-linked variants to uncover mechanisms and validate therapeutic targets.

Ready for hign-content imaging and co-culture.

Optimized for complex screening platforms and multi-cell type systems for advanced phenotypic analysis.

Technical Data & Functional Validation

pixHeps demonstrate superiority over conventional IPSC-derived hepatocyte models.

Principal component analysis (PCA) representing the variance in gene expression between conventional and optimised (pixHep) iPSC-derived hepatocytes across three biological replicates.
Top KEGG pathways illustrated as gene ratio of the differentially expressed genes between conventional and optimised (pixHep) iPSC-derived hepatocytes contributing to each annotated pathway and coloured with a gradient defined by the statistical significance of pathway enrichment.

Maturity Markers

Representative immunocytochemistry pictures showing protein expression of the hepatocyte maturity markers albumin (red), alpha-1-antitrypsin (A1AT; green), HNF4A (green), and AFP (red) in liver carcinoma HepG2 cells, pixlbio pixHep, and primary human hepatocytes (PHH).

Urea Cycle Markers

Urea secretion in liver carcinoma HepG2 cells and pixHeps. Data are presented as mean±SEM of n=3-4 independent experiments.

Functional Membrane Localization of ASGR1

Representative immunocytochemistry images showing the localization of ASGR1 in the pixHep membrane. Cells were counterstained with the membrane marker E-cadherin and DAPI (upper). The effect of ASGR1 in the transport of GalNAc-siRNA conjugate targeting GAPDH in pixHeps using GalNAc-Cy3 staining (bottom left) and qPCR (bottom right). Data are presented as mean±SEM of n=3 independent experiments. mRNA expression data were normalized to 18S rRNA. NTC: non-template control.

Phase I and Phase II Enzyme Activity

mRNA expression levels of Phase I CYP450 genes in liver carcinoma HepG2 cells, pixHeps, and PHHs.
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