pixStellate iPSC-derived Stellate Cells

Human iPSC-derived stellate cells for modeling fibrosis and hepatic inflammation.

Express key fibrotic and activation markers (COL1A1, ACTA2, PDGFRβ)
Respond to TGF-β and LPS stimulation for fibrosis induction studies
Donor-matched Co-culture compatible with pixHep for MASLD and steatohepatitis modelling

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pixStellate™ are iPSC-derived stellate cells that maintain hallmark markers (α-SMA, desmin, GFAP) and activate under fibrogenic stimuli.

They can be used alone or paired with pixHep™ to model fibrosis progression, MASLD-to-NASH transitions, or chronic liver injury.

Quiescent in baseline culture; responsive to injury cues.

Express hallmark stellate markers confirmed via immunostaining.

Upregulate pro-fibrotic genes upon activation.

Available from healthy or disease donor lines.

Compatible with custom genetic engineering

Technical Data & Functional Validation

pixlbio iPSC-derived Hepatic Stellate Cells (pixStellate) express key HSC markers at gene and protein level

mRNA levels of HSC markers (Desmin, PDGFRb, ALCAM, PCDH7, ACTA2, and COL1A1) in pixlbio pixStellate following 2 weeks of iPSC differentiation and primary HSC (pHSC).

Key HSC Markers (mRNA)

mRNA levels of hepatocyte markers (ALB, A1AT, HNF4A) in iPSC-derived pixStellate and pixHep as well as pHSC and primary human hepatocytes (PHH). Data are presented as mean±SEM of n=2 independent experiments. mRNA expression data were normalized to 18S rRNA.

Key HSC Markers (Protein)

Protein levels of the key HSC markers Desmin and GFAP alongside low protein levels of the HSC activation markers Collagen I and αSMA in DefiniGEN Ulti-HSC, indicative of their quiescent status.

Quiescent Phenotype

Accumulation of vitamin A in the form of lipid droplets in DefiniGEN hepatic stellate cells over time, as measured by BODIPY staining.

pixStellate Activation

mRNA levels of the HSC-activation markers COL1A1 and ACTA2 in vehicle-treated, LPS-treated (100 ng/ml), or FBS-treated (10%) DefiniGEN Ulti-HSC for 5 days.

Pro-collagen I secretion levels in vehicle-treated, LPS-treated (100 ng/ml), or FBS-treated (10%) DefiniGEN Ulti-HSC for 5 days. Data are presented as mean±SEM of n=2-3 biological replicates. mRNA expression data were normalized to 18S rRNA.

MASH modeling through innovative co culture of iPSC-derived liver cell systems

Case Study

October 14, 2025

MASH modeling through innovative co culture of iPSC-derived liver cell systems

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