pixHep/pixStellate Co-Culture Models

Multi-cell systems combining pixHep hepatocytes and pixStellate stellate cells for advanced disease modeling.

Recapitulate disease hallmarks of steatosis, inflammation, and fibrosis progression in vitro
Enable mechanistic and compound-response studies in a controlled environment
pixHep (HEP-003, 1 × 10⁶ cells per cryopreserved vial) and pixStellate (HSC-003) optimized for advanced, donor matched co-culture studies (1 cryopreserved vial of each required

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This model recreates the progression from MASLD to fibrotic MASH by combining lipid-loaded pixHep™ with activated pixStellate™ cells.

Under steatogenic and pro-inflammatory conditions, hepatocytes accumulate fat and signal stellates to deposit collagen and upregulate fibrotic genes.

Integrated lipid, inflammatory, and fibrotic readouts.

Quantifiable collagen deposition and α-SMA upregulation.

Compatible with anti-fibrotic, metabolic, and anti-inflammatory drugs.

CRISPR-enabled for genetic risk variant modelling.

Technical Data & Functional Validation

Key HSC and Hepatocyte Markers (mRNA)

mRNA levels of the hepatocyte markers albumin (ALB) and alpha-1-antitrypsin (A1AT) and hepatic stellate cell markers collagen (COL1A1) and Desmin in pixlbio pixHep, pixStellate, and pixHep/Stellate co-cultures.

Hepatic Steatosis and Pharmacological Reversal

Quantification of lipid accumulation in pixHep and pixHep/pixStellate co-culture, as measured by BODIPY staining (left). B) Quantification of lipid accumulation in pixHep/pixStellate co-culture following treatment with either vehicle, Firsocostat (10 μM), Alk5i (5 μM), or combination of Firsocostat and Alk5i for 5 days, as measured by BODIPY staining. Data are presented as mean±SEM of n=2-3 independent experiments.

Fatty Acid Treatment and Collagen Secretion

Quantification of lipid accumulation in pixHep/pixStellate co-culture pre- and post- free fatty acid (FFA) treatment, as measured by BODIPY staining (left). Quantification of lipid accumulation in FFA-treated pixHep/pixStellate co-cultures following treatment with either vehicle, Firsocostat (10μM), Alk5i (5 μM), or combination of Firsocostat and Alk5i for 5 days, as measured by BODIPY staining. Data are presented as mean±SEM of n=2 independent experiments (middle). Collagen secretion in pixHep/pixStellate co-culture following treatment with either vehicle, Firsocostat (10 μM), Alk5i (5 μM), or combination of Firsocostat and Alk5i for 5 days, as measured by ELISA. Data are presented as mean±SEM of n=2-3 independent experiments

MASH modeling through innovative co culture of iPSC-derived liver cell systems

Case Study

October 14, 2025

MASH modeling through innovative co culture of iPSC-derived liver cell systems

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Our pixCell portfolio seamlessly integrates into custom data generation projects—from functional assays (pixCellServices) to phenomic analysis (pixCellPaint)—delivering ready-to-use data at your fingertips.

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